Reinvasion is worst

Last year 2015 was a bad year for the bees in more than one respect. Long cold spring and bad summer. But late summer was good as was the autumn. The bees could recover and prepare for next year.

Fresh pollen was low in spring 2015 . The immune system wasn’t at its best. And the bees couldn’t fly as much as they needed. Where nosema was present it could infect new bees instead of disappear with sick bees as those couldn’t make it back to the hive.

I treated more hives and used more Thymol last year than I had done earlier years. Early in the season I saw what was coming and decided to try some new strategies to get forward on the path to better Varroa resistance and finally get rid of chemicals.

Leif Hjalmarsson

Very often I have reminded myself of the experiences from an apiary of Leif Hjalmarsson in the southern part of Sweden. He got 5 Elgon queens from me already in 1997 and established an apiary at least 2 miles from other bees. The bees to which he introduced the queens had been treated with Apistan for about 5 years and were very low in number of mites. The bees had probably been adapted somewhat to the presence of the mite. Leif used large cell size, 5.4 mm. He never had to use any miticide on those bees until he died early this year. I do miss him! He was dear friend and a good beekeeper.

Already when we started to combine the Monticola bee with our Swedish bees there could be seen resistance traits. Therefore I had hoped that Varroa mites would be no problems when they arrived in my apiaries. This would not be the case. Even though I had as well regressed my bees to small cell size, 4.9 mm.

I have been wondering since 2008 when the mite started to create problems, why they were a problem for my bees with me, but not for “my” bees with Leif? Last year I decided I had to let the fact that Leif Hjalmarsson established this apiary of his more than 2 miles from other bees affect how I designed my bee management. I want of course to get better resistance with my bees in my apiaries.

A lot of focus had to be put on avoiding reinvasion, bees picking up mites from colonies with high infestation level of mites, within my own apiaries and others within about 1.5 miles.

Better selection 2015

But the difficult circumstances for the bees last year also meant that selection pressure was stronger and it was easier to choose a good breeder, if there was any. And there were good breeders, especially colony S241 which I have mentioned in an earlier blog post, but also C243. I introduced many new queens in my colonies last year, especially from S241. I had only found one mite in S241 in the brood when I tried to found out the VSH degree. And that mite had no offspring.

1. Project 1, 3 miles from other bees in a forested area started in autumn 2014. I had not treated the colonies in this project in that autumn when the project apiary was established. During 2015 I treated a couple of colonies when they showed crippled winged bees. The varroa level was not alarmingly high, so virus effects came early in infestation. Evidently reinvasion from the colonies with DWV didn’t occur very much to the other colonies in the project apiary, as in autumn 2015 varroa level was low in all colonies. Varroa level was also very low this spring 2016, and so it is now late in summer 2016.

Bee shaker1 The Bee Shaker is a valuable tool in getting a quick and easy figure of the Varroa level in the bee colonies in an apiary. Type in the search box: Bee Shaker – at the top of this blog, and you will find more info about it.

2. Project 2 started late summer 2015 as I treated all colonies in an apiary 1.5 miles from other bees of mine with an effective pesticide/miticide in August that year (I hated to do use it), to mimic somewhat how the apiary of Hjalmarsson was established. The varroa level now in August 2016 was surprisingly low, almost not detectable. It was in this apiary I had planned to reduce Varroa level by removing all capped brood frames twice with a week in between, in those colonies showing higher Varroa level than 3%. The highest level now in August was 0.3%.

Bee shaker2 The Bee Shaker will help you to decide if any colonies has to be removed (or any other action taken) from your apiary in which you are developing the Varroa resistance in your bees. It’s a simple and quick method, but you kill 300 bees. That is though nothing compared to numerous bees dying if you do nothing. Viruses and mites killing thousands of bees besides the normal die offs from worn out bees in their daily work, where tens of thousands of bees are dying.

3. Those colonies I decided last year I would shift queens in this year, I treated with thymol in April/May this spring even if they showed no signs of Varroa or virus symptoms. With this I think I secured that those colonies wouldn’t produce mites that could reinvade the other colonies in the apiary. These kind of apiaries are the most numerous of mine. I have not checked the varroa level in all colonies spring and late summer in these apiaries.

Up til now in late August 2016 I have found this odd colony in just a few apiaries, showing one wingless bee. I have tested those colonies with the bee shaker for the Varroa level and only found a few colonies with a Varroa level higher than 3 %.

In one apiary I had this colony with a decreasing varroa level during the season. Three weeks after the early August measurement with just above 1 % Varroa level, I made a new measurement. Now it showed 5 %! Too many mites for the colony to have produced it during these three weeks. Less than a mile away there is another beekeeper, with Elgon bees, but not selected for Varroa resistance for some years. No wingless bees. The colony had probably picked up the many mites from one of his colonies. I didn’t treat, but gave it a sticky board on the bottom to check the natural downfall. And a new Varroa level check will be done in a month. Hopefully this colony will be able to reduce the Varroa level. We’ll see.

An apiary at the edge

Let’s look at one apiary at the edge of “my” Elgon area. With that I mean that drones from other beekeepers’ bees can influence the matings of virgin queens. Also the risk of reinvasion is of course higher. Last year was of course not only difficult for my bees but also for the bees of other beekeepers. In this apiary I treated two colonies quite a lot last year, and also this spring and shifted their queens. The other colonies in this apiary has not been treated this year. But they were all treated last year with Thymol. Now in August I saw a bee that looked like it had the beginning of wings being crippled in one of those colonies. So I tested the Varroa level, 4 mites in 350 bees = 1.1 % Varroa level. It got no treatment of course. Very pleasing result.

A couple of small apiaries

I have a couple of small apiaries in which I checked all colonies with the Bee shaker in spring this year, 0 or a couple of mites in 300 bees. No colonies treated this spring as I didn’t plan to shift any queens this year. I had shifted some last year. I checked these apiaries now in August.

In one of the small apiaries with two colonies and a split I found 4 mites in one of the big colonies and 32 (!) in 300 bees in the other. This was too many in the later colony for it to be able to produce them by itself. No crippled winged bees. Also a sign of that those mites were picked up from somewhere else. This colony had a history of needing Thymol every year, so I chose to treat with Thymol and am planning to finally shift the queen next year. 500 meter away is double the amount of hives of another beekeeper (with Elgon bees which were not from my selection in recent years). He had had problems with one of his colonies.

The situation in the other small apiary will be described later in a blogg post of its own.

Avoid reinvasion when bees adapt

The conclusion is that it’s very important to avoid reinvasion of mites when your bees are developing their ability to control the Varroa mites. They need mites to do that, but no or few extra mites from other colnies that makes it more difficult for them to survive.

On the other hand can the explanation for the better varroa resistance with my bees, as it seems, partly be explained by the fact that I shifted quite some queens last year to S241-daughters. Bess from their colonies will drift somewhat into other colonies, as bees from all colonies do. In this case these drifted bees may well help controlling the Varroa level and possibly also teaching the original bees of the colony to fight mites better.

Genetics is of course important when bees adapt, changes in the composition of the DNA. Selection by culling the worst and multiplying the best. But bees adapt too quickly for the genetic changes to explain in all. Epigentic changes is of uttermost importance here, changes how the existing DNA is expressed, how it’s used. It’s impossible to explain the resistance developing in S. America and S. Africa in about 5 years in any other way. As epigenetic changes occur when environmental changes act upon the chemical environment closest to the bees. The precense of the Varroa causes chemical changes in the bees, in the brood cells, etc. These epigenetical changes are inherited to next generations until new environmental changes cause other epigenetical changes. We understand that chemical help against Varroa will hinder the bees’ own control mechanisms to develop fully. There will be a balance act of avoiding all kinds of miticides as much as possible without letting the bee colonies die. Avoiding reinvasion will be very important then helping the bees developing their control mechanisms fully.

As was pointed out in a previous blog post, my bees are held on small cells, which may contribute to the very low overall mite level. The mite level in Leif Hjalmarsson’s apiary he didn’t treat for many years was at least in the beginning when we measured substantially higher. He used large cells.

I’m convinced that when the bees have learnt how to control mites effectively they can handle reinavsion of mites as well, maybe also in larger numbers. I would call that VISH (Varroa Intruder Sensitive Hygiene.:)) I suspect this can take some years. Then they probably sometimes need some reinvasion to keep their skill at a high level.

Learning and teaching

 

Hans-Otto Johnsen was very skilled already in his youth keeping old American cars and trucks going. That skill can be very handy for a commercial beekeeper.

For many years he worked as an expert on explosives, but he got poisoned by nitroglycerine and had to change his job for making a living, so he turned to beekeeping.

At the university

For a number of years he worked as a technician under Prof. Stig Omholt in Norway and at the same time developing his commercial operation. His experience from these years has helped him in developing his Varroa resistant bee stock.

HAns-Otto brood A good brood comb in one of his Norwegian type of combs before he switched to medium Langstroth size.

Quite soon he got to know me and wanted Elgon stock to work with. He imported quite a number of splits from me. He kept track of the Varroa levels in the colonies and stopped using any type of chemical to fight anything in the hives. He wanted his bees to develop their ability to survive, which they did.

Hans-Otto & Ed Ed Lusby and Hans-Otto discussing small cell beekeeping at a fuel filling stop on our way to one of the apiaries of Lusbys’ in the Sonoran desert.

In America

We travelled together several times to America and studied small cell beekeepers and wax foundation producers. Hans-Otto bought equipment and started producing wax foundation, small cell and large cell as well as different sizes of drone foundation. His mechanical and engineer abilities showed themselves to be very useful as he changed and improved the equipment, for example the cooling of the drum for producing rolls of uniform sheet for feeding the plain and foundation rollers. Also the setup of plain and foundation rollers needed according to his opinion more controls of individual speeds for different parts of the production process, which he included in the setup.

Hans-Otto and GAry Dadnt Hans-Otto and Gary Dadant discussing wax foundation production during a visit with Dadant’s in Hamilton.

Research

He started to plan and set up different tests for looking at the effects of different cell sizes in brood combs and to produce virus free drones to mate with virgin queens. He saw that bees easier recognized (and removed) when drone brood was infested with mites when these cells were smaller, which they naturally are with smaller worker brood cells. He also saw that mites more readily infested the biggest drone cells.

He was involved in small cell tests, of his own and together with others. One can be found here: http://beesource.com/point-of-view/hans-otto-johnsen/survival-of-a-commercial-beekeeper-in-norway/

Today Hans-Otto has research money from the Department of Agriculture in Norway.

Resistant stock

He developed his bees in quite isolated areas, but not totally isolated, so sometimes the bees were mated to carniolans, buckfasts and the native brown bee (Mellifera mellifera). He also worked together with Terje Reinertsen, another Norwegian beekeeper, very similar to him when it comes to beekeeping. They exchanged breeding material. Both of them have discovered that their bees teach other bees how to get rid of mites. It seems this ability to teach new bees is very important knowledge when developing a Varroa resistant stock.

Today Hans-Otto hasn’t treated his bees now for at least 12 years. The levels of mites are normally very low in his and Terje’s colonies and he never sees any wingless bees. In 2014 the bees of Terje were tested for Varroa levels by the Norwegian Beekeeping Association in preparation for planned research. (Birøkteren, vol 131, 2015(1), pages 13 and 24. The Bee Journal of the Norwegian Beekeepers Association.) The levels were so low it was difficult to calculate the reproduction rate.

When Hans-Otto moves his bees to the heather in late summer, for producing heather honey, his bees quickly pick up quite some mites. The natural downfall of mites will then be higher until about a month before the frost will make the bees form winter cluster. Then the downfall is almost zero again.

Book contribution

In 2010 Georgia Pellegrini (https://en.wikipedia.org/wiki/Georgia_Pellegrini) published her first book on natural food: Food Heroes (http://www.amazon.com/Food-Heroes-Culinary-Preserving-Tradition/dp/1584798548) She included a chapter about Hans-Otto and his focus on natural production of honey. For example he concludes that small cell bees are more biologically optimized than large cell bees. Thus research done with small cell bees are more reliable concerning what bees are and how they react naturally. In short, research results with small bees are more reliable.

In this context it’s interesting to notify that Norwegian wax is almost pesticide free.

HansOttoJohnsen An important part in his quality control is producing wax foundation as he thinks will be the best help for the bees.

Learning and teaching

Today we understand that adaptation of bees to fighting Varroa isn’t only selection breeding, natural or beekeepers’, for changing the DNA composition, but also epigenteic adaptation, the change of expression of the DNA as a result of changed environmental pressure on the bees. This turns the focus to the importance of locally adapted bee stock. Now research is going on with a third adaptation step, how bees learn how to deal with challenges and how they pass on this knowledge to other bees, worker bees to worker bees.

Hans-Otto caught a carniolan swarm of not resistant large bees that choose one of his swarm traps for their new home. After establishing this swarm in one of his apiaries he shifted its place with one of his resistant colonies. So this nonresistant colony received the field bees of a resistant colony. Afterwards they both behaved like resistant colonies.

One year he bough buckfast virgin queens not selected for Varroa resistance. He put them in splits made from his bees. The virgins mated in his apiaries. These splits were spread out in different apiaries of his. For two years they kept their colonies working fine and resistant to mites as good as his other colonies.

Now these two experiments absolutely are food for thought.

More than15 minutes of fame

Definitely Hans-Otto Johnsen is worthy of more than the 15 minutes of fame, one commentator thought was enough.

The bee shaker and varroa resistance

Skak botten 2lc One mite from 300 bees.

I understand that sometimes it’s a good idea to get an idea of the infestation level of varroa mites in bee colonies. You can take samples from a couple of colonies in an apiary to get an idea when to treat. But my first concern is breeding varroa resistant bees.

I have never monitored the varroa infestation level in my colonies. I haven’t had time and I haven’t found any reason for it because I thought I had found a good compromise – treating with Thymol when I saw wingless bees on the hardboard in front of the hive entrance, checking every 10 days or so.

 

Good results up till now

I give a colony one or two pieces of dish wash cloth containing 5 grams of Thymol each when I see wingless bees crawling on the hard board in front of the hive. But this means I don’t treat every colony at the same time (hopefully some not at all in a season). This results in some colonies with higher mite loads not showing wingless bees yet. So these colony (-ies) will through reinvasion increase mite levels again quite quickly in those colonies recently treated.

But this way I’ve been able to develop more and more resistant bees and still produce a good crop. There have been a number of bees not producing any honey. Winter losses have been reduced from 30 % to 10-15 % (except the first year with varroa trouble when I lost 50 %).

The bees have been better chasing mites and remove infested brood. I’ve got good reports from for example Poland and Germany of low populations of varroa in colonies headed by Elgon queens, compared to other bees. And the VSH trait is becoming better and better. Daughters of my colony with the highest VSH % (80) gave colonies that in Poland dropped 2-5 mites after effective treatment while other colonies dropped more than 1000.

 

Thymol is useful but hinders total adaptation

I now have been aware that by having this regime I have a constant quite high varroa population in the apiaries as a whole, and thus probably a climbing virus pressure. In a way this is good as selection is done also on virus resistance.

How do I know that? Now when I’ve used the bee shaker somewhat this year I’ve seen that colonies may show wingless bees (DWV-virus) at low mite infestation. Such low infestation you didn’t expect them to do so, sometimes even as low as 2 % infestation (a daughter from a colony with high VSH trait [80%]! This experience and others similar, raise the question if very high VSH comes with higher susceptibility to viruses.). Wingless bees at 2 % infestation is totally different from a report I’ve got from a test further down in Europe. (There they normally treat effectively every year.) In that test where they didn’t treat at all, my bees didn’t show any wingless bees at 35% infestation while other bees had a lot.

 

How to explain the high infestation level in the test

Now I have to try to explain why my good bees could arrive at 35 % mite infestation. This is interesting and brings up another topic as well. The importance of memories of the worker bees (their knowledge how to chase mites), not only their genetics (and epigenetic history). My queens in this test down in Europe were introduced to bees that had not been selected the same way as mine, and those bees had been treated effectively every year. The bees could probably not chase mites as well as mine.

But of course the genetics from my queens would more and more influence the workers to build up a better behavior when it comes to chasing mites. When the bees have arrived at a good mite chasing mood they learn new bees born in the colony what they have achieved, more than what just come directly with the genetics. In Norway with Terje Reinertsen and Hans-Otto Johnsen experiences are achieved pointing strongly to this.

In an apiary where many colonies are non-resistant as in this European test, you get a mixture of all bees in the apiary through drifting and robbing. This is taking place more and more when the mite populations in the colonies rise. As it did in this test as the colonies were not treated at all.

In a situation when colonies are receiving a lot of mites from neighboring colonies, even the very best kind of mite chasing behavior is maybe not enough to keep mite levels low.

In the test apiary previous to this test, effective treating every year had kept the mite and virus levels low, so the mite population could grow much in the test without showing wingless bees – like in the beginning when the mite first arrives to an area. Then the virus levels are usually very low and there could be 10 000 mites and more in a colony without any signs of viruses (documented case in Sweden in 1987 when the mites were first detected on the island Gotland in the Baltic).

The bees in this test were on 5.5 mm cell size, while my bees are kept on 4.9 mm.

 

Also Thymol hinders total adaptation

I have talked to some beekeepers whose bees are totally treatment free since many years (Hans-Otto Johnsen in Norway, Richard Reid in Virginia and Myron Kropf in Arkansas). Their bees have now small populations of mites and are showing no wingless bees.

I have come to realize that also Thymol is a chemical that hinders the bees to fully adapt to handling the mites successfully on their own. It is in first place the epigenetic adaptation I have come to think of that is disturbed when alien chemicals (like miticides of all kinds) are present. Epigenetic changes take place when a chemical change occur due to environmental changes, like for example the presence of the mite. (But it should be said also that if you use Thymol regularly spring and late summer in a system not selecting better bees like I do, winter losses can be kept low. I know because beekeeper friends do like this.) Also Thymol like other miticides is lowering the immune system of the bees.

How do I then integrate these insights to go further in becoming totally treatment free?

 

A new strategy to try

I’m planning a new strategy, at least to start with in one quite isolated apiary. I have to stop using Thymol. First though I think I have to knock down the mites effectively to reduce the virus level. And then get a better control of the number of mites and take action without any chemicals if varroa populations are rising too much in colonies.

 

The role of the bee shaker

Here the bee shaker will play a role. And I have looked more into how Randy Oliver uses it. It’s much easier to shake a frame of bees into a bowl or pan and then with a measuring cup scope somewhat more than a deciliter (3.5 oz) of bees and pour them into one half of the bee shaker, half filled with alcohol (for example methylated spirit or rubbing[isopropyl] alcohol). Then screw it together and shake for a minute before reading the result. Compared to holding the bee shaker close to a frame side with bees and pour bees into it moving it slowly upwards, the alternative of Randy Oliver is quicker (at least for me). The next step is to test the VSH trait in the best colonies.

Skak yngelrum Start checking from one side in the uppermost box with brood. The queen hopefully will run to the brood if she is outside the brood area (probably not). The comb closest to brood you check so the queen is not there. 

Skak deciliter Shake the bees into a pan or bowl. Scope up somewhat more than a deciliter of bees (3.5 oz)

Skakburk sprit Pour the bees into one of the halfs of the bee shaker, which is half filled with alcohol. Screw the other half tight on top. Shake it for a minute.

Skak botten1lc Turn the shaker upside down and continue shaking until all the alcohol has poured down. Lift it up against the sky and count the mites. This colony had 14 mites on 300 mites and it got two pieces with 5 gram Thymol each. It showed no wingless bees.

What I hated to do

So what I’ve done so far is something I hated to do. In one quite isolated apiary I used an effective chemical miticide (only this time I promised myself) in August 2015. I wanted to use something else than Thymol to give the bees a break from that chemical. And I wanted to knock down the mite population effectively to lower the virus pressure in the apiary. I collected the knocked down mites. (In the rest of the apiaries I plan at the moment to continue as before.) The colonies that had needed most Thymol earlier in the season had the highest downfall of mites. They got probably continuously reinfested from other colonies that happened to not show wingless bees while they anyway had quite high mite loads. The defense system of these quickly reinfested colonies was probably lowered by Thymol, which made this relatively quick reinfestation possible.

One colony that hadn’t needed any Thymol at all (and very little the year before) and still had given me 80 kg (175 lb) of honey with 20 kg (45 lb) left for winter dropped less than 200 mites. And this happened in this very bad season of 2015. This colony is of course a breeder for the coming season.

 

The new strategy

Next year I will in this new strategy apiary make splits from the best colonies and place them in the same apiary (or if the number is enough there, place in other apiaries). In the least good colonies in this apiary (those with highest infestation level) I will kill the queen and give them a ripe queen cell bred from a good colony in this apiary. I check the number of varroa (infestation level) with the bee shaker twice a season in all colonies in this apiary. Each time it will take about 5 minutes per colony. And I look for eventual wingless bees on the hard board in front of the entrances. Before the number of mites rise too high (whatever that is), or when I see wingless bees, I plan to remove all capped brood (worker and drone brood) once or twice with a week in between. I haven’t decided what to do with those brood frames yet. Any suggestion?

 

 

Treatment free feral bees

Up till now anyway, this colony of bees (and their ancestors forming this colony’s ancestor colonies) that has lived in a wall since several colony generations, has never been treated with any kind of chemicals ever, against Varroa mites or anything else.

June 29 last year I caught a swarm that came from this wall in a non-heated old house. (http://www.elgon.es/diary/?p=515) Towards the outside of the wall from the bees they had no insulation whatsoever. Just a thin board of wood. At the inside though a thick log wall.

For a couple of years there’s been an Elgon apiary 3 km away (2 miles). But the bee colony has been longer than that in the wall. Further back in time the closest apiary was 6 km (4 miles) away. At that time the Varroa mite had not arrived to these bees. For many years this colony has swarmed every year.

The swarm I caught was not big, but it had an egglaying queen and built up strength well enough to winter safely. To help it make a lot of brood I provided it with a shallow super above an excluder. I shouldn’t have done that I think as it was too easy for me to just take away this honey super when it was time to prepare the colony for winter. That is stressful time.

Now the bees hadn’t much honey left so I gave them 20 kg (44 pounds) of sugar in sucrose solution. If I hadn’t taken the small amount of honey it would have had about 10 kg (22 pounds) of honey for winter storage. Seeing how the colony behaved I think it would have made it well through winter with that amount. My first colony ever in 1974 had about that amount its first winter.

I saw no wingless bees during the season last year, so they got no Thymol against mites. I didn’t then have any quick way to measure the mite population (but here is at least one: http://www.elgon.es/diary/?p=354) And as I mentioned it was stressful times for me.

The queen stopped laying entirely in late summer. In November I saw through the plastic sheet used as kind of inner cover that the bees was sitting tight together like vacuum-packed peanuts.

FeralWIntered

About 10 March this year when the bees had their main cleansing flight after winter the cluster had spread out and filled more room than in November. It was very few dead bees on the bottom board. And not one defecate spot at the entrance.

These bees seems at least to be more winter hardy and be more Varroa resistant than common beekeepers’ bees, which have not been selected for Varroa resistance.

 

A possible scenario

A swarm of Elgon bees flying from the Elgon beekeeper 6 km away finds the cavity in the wall. Varroa mites havn’t arrived yet to the area. No beekeeper robs the honey or exchanges it for sugar. The cavity is not bigger than maximum two big boxes a beekeeper uses. The amount of brood can’t be as big as in a beekeepers hive. And the restricted area makes the volume finally too small for the bees (no beekeeper puts on boxes) and they swarm, every year mostly. Insulation is almost none. No beekeeper renews the wax and the bees build what they want when it comes to for example cell sizes. The Elgon beekeeper used small cell size to begin with. Here the cell sizes may become still smaller due to cocoon residues.

The bees adapt to the new environment now when they are on their own, like they were before there were any beekeepers around. In this adaption process the epigenetic process is most important, at least at first. The different environment created by a different “hive”, different food (more natural) and different cell sizes (also still smaller) gives a different chemical environment of many aspects. For example the different cell sizes give somewhat different food for the larvae, amount and probably composition also. This results in switching off some genes and turning on others in the DNA. Disturbing chemicals like pesticides and treatments in the hive can hinder this epigenetic process. But not for this swarm. It lived in a non-farming area and no beekeeper put chemicals in their hive.

There were no neighbor bees. Thus no bad influences from non-resistant bees drifting into their hive and no reinvasion of mites.

When the Varroa mites arrived the drones that became “fathers” were those that the mites didn’t parasitize. Maybe they avoided those drone larvae. And also those drones that were parasitized but were not as affected as others, became “fathers”. Thus also an adaption for resistance took place with a change of the DNA. Natural selection thus took place.

As the colony swarms every year there is a break in the brood production. This hinders the reproduction of mites. Also there is both an epigenetic and a genetic adaption with the new generation.

The smaller cells give less attractive food for the mites. They get less fertile on larvae in smaller cells. http://www.elgon.es/diary/?p=596

Drone cells get smaller in colonies on smaller worker brood cells. With smaller worker brood cells you get worker bees that get more hygienic. http://medycynawet.edu.pl/index.php/component/content/article/336-summary-201412/5234-summary-med-weter-70-12-774-776-2014 or http://alturl.com/a8scb Small cell beekeepers, including me, reports a widespread occurrence of uncapping and chewing out of capped brood in both worker and drone brood parasitized by mites. http://www.elgon.es/diary/?p=544 But VSH is said sometimes to not occur on drone brood. But those bees are kept on large cells. At least it doesn’t occur as much in drone brood. But it is observed quite a lot sometimes in small cell colonies as mites are observed to be much more common there in drone brood than in worker brood. http://resistantbees.com/blog/?page_id=2471

 

What happen with feral bees in a beekeeper’s hive?

If a swarm from feral bees end up in a beekeeper’s hive with large cell size, the environment changes and a “reverted” epigenetic process takes place. If there are more bee colonies in the apiary or close by all bees will be drifting (as is common) in all colonies and be mixed more or less. If these other bees have no or very little resistance against Varroa they will have a negative impact on the more resistant feral bees. These were enough resistant in the wall. Are they enough resistant now in this beekeeper’s hive? Maybe not.

If the feral swarm ends up in a beekeeper’s hive with small cell size, and there are neighboring bees that have substantial resistance against the mite, it may be that this swarm will do very well fighting the mites. Especially if there are no or very few bees around that can’t make life miserable for Varroa mites.

What will happen with my feral bees? Will they continue to be treatment free?

MT-colony conclusion

I have shared the performance of this colony which had almost a box of plastic small cell frames and natural positioning of these frames (as the uppermost broodbox). Which also had a tough experience with mice living in the bottom box during winter.

It gave top crop the first crop of winter rape, dandelions and some raspberry. It showed no wingless bees this year early on as it did last year. But it had an old queen. So the colony decided to shift it’s queen and did. Now they showed a few wingless bees. I concluded that was due to the declining amount of open brood to enter for the mites, son inte last brood of the old queen there was enough concentration of mites to develop some wingless bees. But to be consistent with my way of working I gave the colony 9 grams (two pieces) of thymol dish pieces. Next time no wingless bees.

My impression is that the colony is not performing less good with plastic small cell and natural positioning. Thus the conclusion is that plastic small cell frames are not negative for the bees, neither what I call natural positioning. If any of these configurations are positive is difficult to say. An overall smaller mite pressure in the apiary and the area could be the explanation. Due to epigenetic changes that have improved the bees, or/and conventional selection has done its job with the genepool in the apiary/area. Also plastic small cell frames and natural positioning may have contributed. At least plastic small cell may have good influence as there are more cells for each comb, thus faster buildup.

Plastic positioning and the mouse

You remember the previous post about the “multiple test”(MT)-colony, natural positioning, plastic frames, a mouse (or mice), mild winter and what a good condition this colony came out with now in spring. I’ve been thinking about it.

Mild winter

Yes the mild winter has contributed to the good condition. But what about varroa and viruses? Mild winter doesn’t help if you have too much of both in a colony. I would say maybe the contrary, or at least questionable. A lot of varroa triggers extensive brooding, to kind of replace the affected sick brood resulting in a bad rat race.

But less treatment

In fact one of the colonies in the apiary died during winter due to what looked like virus problems, in spite of some more treatment than the good colony got. And another colony which hadn’t received any treatment last year because it didn’t need it, it seemed, no DWV-bees, strong and healthy look – came out with fist size cluster and asking for survival until fresh pollen and steady temp around 60F (15C). We’ll see.

And this good colony got less Varroa treatment 2013 compared to 2012, actually half – at the most only a tenth compared to non-selected bees for Varroa resistance. With the same queen 2012 and 2013.

 Weaker varroa pressure

Now I think in spite of the dead colony and the one with the fistsize cluster, the average Varroa pressure, total amount of mites in the apiary, was less in 2013 compared to 2012. In fact I think this is the case in all my apiaries. Because I used in average about half the amount of thymol I used in 2012. This good colony I’m talking about specifically now got the average amount, 5 grams. Also, only 50% of the colonies going into winter had been treated during 2013, compared to 80% 2012. And, this spring winter die off will be less than 5% compared to more than 15% last spring.

The Varroa pressure I think is lower due to better Varroa resistance in average in my stock, also in this apiary. The least good ones are showing up every spring, either they die in spite of treatment, or they show up weak and get treatment and later their queens are shifted.

 Epigenetics and social learning

Also, from other beekeepers experiences, after about 5 years, mite pressure becomes weaker without any obvious reason. Probably some kind of epigenetic adaption (genes turned on and others turned off) is taking place that takes about 5 years from the first time the mites created problems. This spring is the fifth since the mites gave big problems the first time in all my apiaries.

This adaption to the presence of the mite and fighting it probably also involves social learning. Bees learning to handle the mite and pass this knowledge on to other bees in the hive (http://www.hindawi.com/journals/psyche/2013/768108/). Therefore it’s important not only to propagate good resistant bee colonies by making new queens from it, but also by making new bee colonies from it, including worker bees that can pass their knowledge to new generations of bees.

Plast49Yngel

 Uniform small cells

From the dead colonies through these diffcult years with Varroa I have harvested quite some badly drawn small cellsize combs. With too many patches of drone comb, and too many patches of bigger sometimes irregular “worker” cells. Good they have been culled.

These plastic frames with plastic foundation with cellsize about 4.95 from Mann Lake (http://www.elgon.es/diary/?p=119) that was used in the bigger part of the third (the uppermost) broodbox in the MT-colony at least didn’t have a big negative impact on the colony health. Plastic haters might draw such a conclusion right away. Now, I’m not a plastic lover. I don’t like using plastic. That’s because in general it isn’t degradable in nature. That’s what I want, degradable plastic. But here it is in our world and I tried it.

These plastic frames might even have contributed to the good health of the MT-colony. 10% more cells per comb, still quicker build up. No drone patches (BUT there should be drone patches here and there in the brood area to catch the mites that are there), no patches of bigger and irregular “worker” cells. No wavy combs (I use no wiring in my wax combs). No big bridges of hindrance of wood for the queen between brood boxes.

So I plan to try some more plastic frames from Mann Lake in brood boxes. But there should be a good balance between plastic and wooden frames as long as I will be using the plastic.